Reference board

Philipp L-M¹ et al.

¹Institute for Experimental Cancer Research, Kiel University, University Hospital Schleswig-Holstein (UKSH), Campus Kiel, Germany

KEYWORDS:

Pancreatic adenocarcinoma; PDAC; Cancer stem cells; EMT; Epithelial–mesenchymal-transition; Heterogeneity; Plasticity; Adhesion; Migration; Invasion; Metastasis

DOI:10.3390/cancers16040686

Holthaus D¹ et al.

¹Department of Gynaecology and Obstetrics, University Hospital Schleswig-Holstein, Kiel, Germany.

KEYWORDS:

ADAM17; Cervical organoids; Live cell imaging; Cancer research, SYBOT X-1000; CYTOMAT 2 C-LiN; Lab automation; Fluorescence imaging 

DOI:10.1101/2024.01.27.577547

Ullrich V^1,2,3,4 et al.

¹DKFZ-Division Translational Neurooncology at the WTZ, DKTK partner site, University Hospital Essen, 45147 Essen, Germany.
²German Cancer Consortium (DKTK), partner site Essen/Düsseldorf, a partnership between DKFZ and University Hospital Essen, Germany.
³West German Cancer Center (WTZ), University Hospital Essen, 45147 Essen, Germany.
⁴German Cancer Research Center (DKFZ), 69120 Heidelberg, Germany.

KEYWORDS:

Cancer; Cell biology; Health sciences; Pharmacology; Glioblastoma; Temozolomide (TMZ)

DOI:10.1016/j.isci.2023.108596 

Chakrabarti L¹ et al.

¹Cell Culture & Fermentation Sciences, BioPharmaceuticals Development, R&D, AstraZeneca, Gaithersburg, USA.

KEYWORDS:

Cell line development (CLD); Flow cytometry; Single-cell cloning (SCC); Monoclonality; mAB; CHO suspension cell line

DOI:10.1002/btpr.3441

Dodkins R¹, Delaney JR¹, Overton T², Scholle F², Frias-De-Diego A³, Crisci E³, Huq N⁴, Jordan I⁵, Kimata JT⁶, Findley T¹, Goldberg IG
¹ViQi Inc., Santa Barbara, CA, 93117, United States. 
²Department of Biological Sciences North Carolina State University Raleigh, NC 27695, United States.
³College of Veterinary Medicine, Department of Population Health and Pathobiology, North Carolina State University, Raleigh, NC 27695, United States.
⁴Melbec Microbiology Ltd, Rossendale, Lancashire, BB4 4QJ, United Kingdom.
⁵ProBioGen AG, Goethestr. 54, 13086 Berlin, Germany.
⁶Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX, United States.

KEYWORDS:

Label-free; Viral infectivity assay; Antiviral screening; Brightfield microscopy; Cell-based assays; Machine learning; Convolutional neural networks

DOI:10.1016/j.slast.2023.07.003

Kebir S^1,2,3,4 et al.

¹DKFZ-Division Translational Neurooncology at the WTZ, DKTK Partner Site, University Hospital Essen, Essen, Germany.
²German Cancer Consortium (DKTK).
³Division of Clinical Neurooncology, Department of Neurology, University Hospital Essen, Essen, Germany.
⁴West German Cancer Center (WTZ), University Hospital Essen, Essen, Germany.

KEYWORDS:

Therapy resistance; rare ALDH1A1+ tumor cells; Temozolomide (TMZ); Glioblastoma; ALDH; DEAB

DOI:10.1158/1078-0432.CCR-22-0611

Hugendieck G¹ et al.

¹Department of Gynaecology and Obstetrics, University Hospital Schleswig-Holstein Campus Kiel, Germany.

KEYWORDS:

ADAM17; Ascites; Chemoresistance; Extracellular vesicles; Ovarian cancer; Single cell cloning (SCC); Cancer research 

DOI:10.1002/jev2.12338 

Heinzelmann D¹ et al.

¹Bioprocess Development Biologicals, Cell Line Development, Boehringer Ingelheim GmbH & Co. KG, Birkendorfer Strasse 65, 88397 Biberach, Germany.

KEYWORDS:

Cell bank characterization; Cell line development; Difficult-to-express protein; Droplet digital PCR; Genetic characterization; Prediction of molecule assembly, CHO-K1; Monoclonality

DOI:10.1016/j.nbt.2023.10.001 

Gurzeler LA¹ et al.

¹Department of Chemistry, Biochemistry and Pharmaceutical Sciences, University of Bern, Switzerland

KEYWORDS:

eRF1 degradation; genetic diseases; nonsense mutation; NVS1.1; NVS2.1; CFTR; IDUA; Cystic fibrosis; Hurler syndrom; Ribosome-associated quality control; SCC

DOI:10.1101/2023.01.31.526456

Nagy A¹ et al.

¹Cell Culture and Fermentation Sciences, Biopharmaceutical Development, BioPharmaceuticals R&D, AstraZeneca, One MedImmune Way, Gaithersburg, MD, 20878, USA. 

KEYWORDS:

Engeneered CHO cells; Gene therapy; Single cell cloning (SCC); HSV-based platform

DOI:10.1038/s41598-023-46298-3 

Wolf C¹ et al.

¹Department of Pediatrics, Medizinische Fakultät Carl Gustav Carus, Technische Universität Dresden, Germany.

KEYWORDS:

CARD8; DPP9; IL-18; IL-1β; Inborn error of immunity; NLRP1; Autoinflammation; Hemophagocytic, Lymphohistiocytosis; Inflammasome; Proinflammatory cytokines. 

DOI:10.1016/j.jaci.2023.07.013 

Dinić J¹ et al.

¹Department of Neurobiology, Institute for Biological Research "Siniša Stanković"-National Institute of the Republic of Serbia, University of Belgrade, Serbia.

KEYWORDS:

ABCB1; ABCC1; ABCG2; MDR markers; NSCLC; Immunofluorescence assay; Lung cancer; Multidrug resistance; Primary cell cultures; Image cytometry

DOI:10.3390/diagnostics13243617

Geisen R¹ et al.

¹SYNENTEC GmbH, Elmshorn, Deutschland

KEYWORDS:

Artificial intelligence (AI); Machine learning; Image analysis; Monoclonality; CLD 

DOI:10.1007/s12268-023-2019-3

Samarin J¹ et al.

¹Cancer Drug Development, German Cancer Research Center (DKFZ), Heidelberg, Germany.

KEYWORDS: 

Tumor resistance; Dose-limiting toxicity; Specific biomarkers; Lung cancer cell lines; “antioxidant-capacity” biomarkers (ACB); STAT3; STAT5A/B; Reactive oxygen species (ROS); Nitric oxide; NYONE

DOI:10.1101/2023.01.17.524372

Veys C¹ et al.

¹Normandie Univ., UNICAEN, BIOTARGEN, Caen, France.

KEYWORDS:

Bcl-XL; Apoptosis; Chondrosarcoma; miR-342-5p; miR-4270; Osteosarcoma; Cancer research; Cell morphology; miRNA mimic screening

DOI:10.1016/j.ajpath.2022.12.009 

Tishina S¹ et al.

¹Department of Translational Genomics, Medical Faculty, University of Cologne, Germany. 

KEYWORDS:

Apoptosis; BCL-2 family; Cancer; Inflammation; Neurodegeneration; Viability; Cytotoxicity; Cancer research;Pancreativ neoplasia

DOI:10.21203/rs.3.rs-2971504/v2

Dong J^1,2 et al.
¹Laboratory of Infection Oncology, Institute of Clinical Molecular Biology, Christian-Albrechts-Universität zu Kiel and University Hospital Schleswig-Holstein, Germany.
²Department of Molecular Biology, Max Planck Institute for Infection Biology, Berlin, Germany.

KEYWORDS:

Cervical cancer; Ectocervix; Human papillomavirus; Immunotherapy; Organoids; γδ T cells; SYBOT X-1000; CYTOMAT 2 C-LiN; Lab automation; Fluorescence imaging 

DOI:10.3389/fimmu.2023.1281646 

Hayford CE¹ et al.

¹Chemical and Physical Biology Graduate Program, Vanderbilt University School of Medicine, Nashville, TN, USA.

KEYWORDS:

Drug tolerance; Idling; Low-low; Melanoma; Metabolism; Metabolic adaptation; Cancer cell subpopulations; Black, clear-bottom 96-well plate (Falcon);  Nuclear count; Ferroptosis-induction experiments

DOI:10.1101/2022.02.03.479045 

Dodkins R¹ et al.

¹ViQi Inc., Santa Barbara, CA, United States.

KEYWORDS:

High-throughput imaging; Viral Infectivity; Automated brightfield microscopy; 20x magnification; Machine Learning; Ai; Viral vaccines; Antiviral therapies; Manufacture of biologicals

DOI:10.1101/2022.03.23.485512

Barzadd MM¹ et al.

¹KTH - Royal Institute of Technology, School of Engineering Sciences in Chemistry, Biotechnology, and Health, Dept. of Protein Science, Stockholm, Sweden.

KEYWORDS:

High-throughput imaging; Aggregation; Autophagy; Bispecific antibody; CHO cells; ER stress; System biology; Trypan blue viability

DOI:10.1016/j.nbt.2022.01.010 

Lutter G^1,2 et al.

¹Department of Cardiovascular Surgery, University Hospital Schleswig-Holstein (UKSH), Kiel, Germany.
²German Centre for Cardiovascular Research (DZHK), Partner Site Hamburg/Kiel/Lübeck, Hamburg, Germany.

KEYWORDS:

Stem cells; ECFCs; Human endothelial colony-forming cells; PCL nanofiber plates; Scaffolds; Biodegradable; heart valve; Human induced-pluripotent stem cells-derived MSCs; iMSCs; Tissue engineering; Automated fluorescence microscopy

DOI:10.3390/ijms23010527 

Jaroš P¹ et al.

¹Department of Biochemistry and Microbiology, University of Chemistry and Technology, Prague, Czech Republic.

KEYWORDS:

Candida albicans; C. krusei; C. parapsilosis; Biofilm; Boswellic acid; Fluconazole; MTT assay 

DOI:10.3390/microorganisms10010171 

Huhn S¹ et al.

¹Process Cell Sciences, Biologics Process R&D, Merck & Co., Inc., Kenilworth, NJ 07033, USA.

KEYWORDS:

Biotechnology; Evolutionary mechanisms; Genetics; Omics; High-throughput imaging; Cloning; 96-well plates; Limiting dilution; Confluence

DOI:10.1016/j.isci.2022.104074 

Beumers L¹ et al.

¹Division of Molecular Genome Analysis, German Cancer Research Center (DKFZ), Heidelberg, Germany.

KEYWORDS:

Barcoded cell lines; Cancer research; f.sight™ FL, Single cell dispensing; Proof of monoclonality; Colony outgrowth; High-throughput; Automated imaging

APPNOTE: www.cytena.com

Anna-Christina Rambow A-C^1,2 

¹Department of Gynecology and Obstetrics, University Hospital Schleswig-Holstein (UKSH), Campus Kiel, Germany.
²Institute for Experimental Cancer Research, University of Kiel, Germany.

KEYWORDS:

Bcl-xL; TRAIL; TRAIL-R4; Apoptosis; Breast cancer; Non-apoptotic signaling; Crystal violet; Pancreatic cancer, Colony growth, Live/dead imaging

DOI:10.3389/fcell.2022.942718 

Chang M¹ et al.

¹Biologics Process R&D, Merck & Co., Inc., Kenilworth, New Jersey, USA.

KEYWORDS:

Chinese hamster ovary; CHO; DNA methylation; Epigenetic regulation; Mammalian biothechnology; Transcriptomics

DOI:10.1002/bit.28036 

Flörkemeier I^1,2 et al.

¹Department of Gynecology and Obstetrics, Kiel University and University Medical Center Schleswig-Holstein Campus Kiel, Germany.

²Pharmaceutical Institute, Department of Pharmaceutical and Medicinal Chemistry, Christian-Albrechts-University Kiel, Germany.

KEYWORDS:

Cancer research;  2D-imaging, 3D-imaging, Apoptosis; Breast cancer; Drug development; Dual topoisomerase inhibitor; CellTox Green; UF-182 primary cells

DOI:10.3390/cancers14010002 

Hagelund S¹ & Trauzold A¹

¹Institute for Experimental Cancer Research, University of Kiel, Germany.

KEYWORDS:

TRAIL; TRAIL receptor; High-throughput fluorescence imaging; Apoptosis; Extracellular pH; Non-apoptotic signaling; Pancreatic ductal adenocarcinoma; Live cell imaging; Navitoclax; Venetoclax; Colony formation assay; Crystal violet

DOI:10.3389/fcell.2022.768579 

He Q¹ et al.

¹Global Product Development and Supply, Bristol Myers Squibb Company, Devens, MA 01434, USA.

KEYWORDS:

Chinese hamster ovary cells; CHO; Clonality; Monoclonal; Master cell bank (MCB); Research cell bank (RCB); Biologics manufacturing; Cell culture platform; Re-cloning; mAB titer

DOI:10.3390/bioengineering9040173 

Fischer S¹ et al.

¹Boehringer Ingelheim Pharma GmbH & Co. KG, Cell Line Development, Bioprocess Development Biologicals, Biberach/Riss, Germany.

KEYWORDS:

Cell line development; CLD; Chinese hamster ovary cells; CHO; Monoclonal antibody; mAB; Proof of monoclonality; High-throughput imaging; N-glycosylation; microRNA; Sialylation; FASCC

DOI:10.1002/bit.28015 

Chakrabarti L¹ et al.

¹Cell Culture & Fermentation Sciences, BioPharmaceuticals Development, R&D, AstraZeneca, Gaithersburg, MD, USA.

KEYWORDS:

CHO; Cell line development; Cell sorting; enrichment; Mitochondrial membrane potential; Productivity; Proteomics; Colony outgrowth measurement; Confluence per well; 384-well plates

DOI:10.1080/19420862.2021.2020081  

Gundlach J-P¹ et al.

¹Department of General, Visceral-, Thoracic-, Transplantation- and Pediatric Surgery, University Medical Center Schleswig-Holstein (UKSH), Campus Kiel, Germany.

KEYWORDS:

Cell migration; Automated wound healing assay; Gap closure; High-throughput imaging; Schwann cells; Cancer assiciated fibroblasts; Cholangiocellular carcinoma; Fluorescence; CellTracker

DOI:10.3390/jcm11102785

Wandishin CM¹ et al.

¹Department of Biochemistry, Vanderbilt University, Nashville, TN USA.

KEYWORDS:

DIP rate; Cell viability; Continuous assay; Drug response; Drug screening; Quantitative analysis; Real-time luminescence; Suspension cells; Red fluorescent protein mRuby2

DOI:10.1080/15384047.2022.2065182 

Schmieder V¹ et al.

¹Cell Line Development, Bioprocess Development Biologicals, Boehringer Ingelheim Pharma GmbH & Co. KG, Biberach an der Riss, Germany.

KEYWORDS:

Acceleration; COVID19; Cell line development; CLD; Chinese hamster ovary cells; CHO; Monoclonal antibody; mAB; Transposase; Proof of monoclonality, Cell growth; High-throughput imaging

DOI:10.1016/j.jbiotec.2022.03.010 

O'Brian NA¹ et al.

¹Division of Hematology/Oncology, Department of Medicine, David Geffen School of Medicine at UCLA, Los Angeles, California.

KEYWORDS:

HER2-Positive Cancer; High-throughput platform; Breast cancer; ER/ ER+/HER2+; Tucatinib; Cell imaging

DOI:10.1158/1535-7163.MCT-21-0847 

Hedemann N¹ et al.

¹Department of Gynecology and Obstetrics, Kiel University and University Medical Center Schleswig-Holstein, Germany.

KEYWORDS:

3D model; ADAM17; Chemotherapy resistance; Combined therapy; Drug testing; Multicontent readout; Ovarian cancer; Primary cells; Primary spheroids; Spheroids; Caspase3/7 activity; Celltox^TM Green

DOI:10.3390/cancers13092039

Hayford C E¹ et al.

¹Chemical and Physical Biology Graduate Program, Vanderbilt University School of Medicine, Nashville, TN, USA.

KEYWORDS:

Tumor heterogeneity; Drug-response; Mutated oncogene; Cancer research; Targeted therapy response; Phenotypic states; Nuclei count; Cell state variability; Drug response variability; Population-level DIP rate assay

DOI:10.1371/journal.pbio.3000797 

Groot J^1,2 et al.

¹Genome Technologies and Computational Sciences, Biogen, Cambridge, Massachusetts, USA.
²Inzen Therapeutics, Cambridge, Massachusetts, USA.

KEYWORDS:

CHO cells; Bioprocess engineering; Cell line development; CLD; next-generation sequencing; NGS; Sequence variant analysis; Fed-batch process; Top candidate clones

DOI:10.1002/biot.202000548 

Tsai C¹ & Sinkala E¹ 

¹cytena GmbH, Neuer Messplatz 3, Freiburg, Germany

KEYWORDS:

Cell line development (CLD); Protein production; Recombinant protein yield; Cell growth monitoring, PAIA Biotech kit (PA-104); Titer measurements; Large-scale manufacturing; c.bird; microbioreactor 

APPNOTE: www.cytena.com

Gross T¹ et al.

¹Laboratory for MEMSApplications, IMTEK-Department of MicrosystemsEngineering, University of Freiburg, Georges-Koehler-Allee103, D-79110 Freiburg, Germany

KEYWORDS:

CRISPR/Cas9; f.sight; Cytena; Single-cell isolation; Gene editing; hTERT MSCs; Osteogenic differentiation; Transfection efficiency; High resolution; High-throughput; Fluorescent single cell cloning (FASCC); Colony growth monitoring; FACS; Limited dilution

DOI:10.1101/2020.08.17.253559

Ohta H^1,2, Yabuta Y^1,2, Kurimoto K^2,3, Nakamura T^1,2, Murase Y^1,2, Yamamoto T^1,4,5,6, Saitou M^1,2,4

¹Institute for the Advanced Study of Human Biology (ASHBi), Kyoto University, Japan.

²Department of Anatomy and Cell Biology, Graduate School of Medicine, Kyoto University, Japan.

³Department of Embryology, Nara Medical University, Japan.

⁴Center for iPS Cell Research and Application (CiRA), Kyoto University, Japan.

⁵AMED-CREST, AMED, 1-7-1 Otemachi, Chiyoda-ku, Tokyo, Japan.

⁶Medical-risk Avoidance based on iPS Cells Team, RIKEN Center for Advanced Intelligence Project (AIP), Kyoto, Japan.

KEYWORDS:

In vitro expansion; PGC-like cells (PGCLCs); Cyclosporin A; Epigenetic reprogramming; Fibroblast growth factor; Primordial germ cells (PGCs). 

DOI:10.1093/biolre/ioaa195 

Tejwani V¹ et al.

¹ Biotechnology R&D, Clone Development Team, Lupin Limited, Pune, India.

KEYWORDS:

Automation; Cell line development; CLD; Monoclonality assurance; Proof of monoclonality; High-throughput; Clone screening; Productivity screening; Single-cell cloning; SCC

DOI:10.1002/btpr.3208 

Cuello F^1,2, Knaust AE^1,2, Saleem U^1,2, Loos M^1,2, Raabe J^1,2, Mosqueira D^3, Laufer S^1,2, Schweizer M⁴, van der Kraak P⁵, Flenner F^1,2, Ulmer BM^1,2, Braren I^2,6, Yin X⁷, Theofilatos K⁷, Ruiz-Orera J⁸, Patone G⁸, Klampe B^1,2, Schulze T^1,2, Piasecki A^1,2, Pinto Y⁹, Vink A⁵, Hübner N^8,10,11,12, Harding S¹³, Mayr M⁷, Denning C³, Eschenhagen T^1,2, Hansen A1^,2

¹Department of Experimental Pharmacology and Toxicology, University Medical Center Hamburg-Eppendorf, Germany.
²German Center for Heart Research (DZHK), Kiel, Germany. 
³Division of Cancer & Stem Cells, Biodiscovery Institute, University of Nottingham, UK. 
⁴Electron Microscopy Unit, Center for Molecular Neurobiology Hamburg, University Medical Center Hamburg-Eppendorf, Germany. 
⁵Department of Pathology, University Medical Center Utrecht, Utrecht University, Utrecht, The Netherlands.Experimental Therapeutics, Mayo Clinic Center for Regenerative Medicine, Rochester, MN, USA. 
⁶Vector Core Unit, University Medical Center Hamburg-Eppendorf, Hamburg, Germany. 
⁷King's British Heart Foundation Centre of Research Excellence, King's College London, London, UK. 
⁸Cardiovascular and Metabolic Sciences, Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany. 
⁹Department of Experimental Cardiology, Academic Medical Center, Amsterdam, The Netherlands. 
¹⁰DZHK (German Centre for Cardiovascular Research), Berlin, Germany. 
¹¹Charité -Universitätsmedizin, Berlin, Germany. 
¹²Berlin Institute of Health (BIH), Berlin, Germany. 
¹³British Heart Foundation Centre of Research Excellence, NHLI, Imperial College London, London, UK. 

KEYWORDS:

Disease modeling; High-content imaging; Endoplasmic reticulum; ER; engineered heart tissue; human-induced pluripotent stem cells; Mitochondria; Phospholamban p.Arg14del; Human induced pluripotent stem cell-derived cardiomyocytes; hiPSC-CM; Mitochondrial DNA; Nuclei count; High-throughput; CRISPR/Cas9

DOI:10.15252/emmm.202013074 

Huhn SC¹ et al.

¹Biologics Upstream Process Development, MRL, Merck & Co., Inc., Kenilworth, New Jersey, USA.

KEYWORDS:

Chinese hamster ovary cell; CHO; DHFR; CLD; Glutamine synthetase; CRISPR-Cas9; Knockout; Transfection efficiency; Zinc finger nuclease; Host cell line; High producer

DOI:10.1002/btpr.3185 

Otto L¹ et al.

¹Institute for Experimental Cancer Research, Christian-Albrechts-University Kiel (CAU) and UKSH Campus Kiel, Germany.

KEYWORDS:

Pancreatic cancer; Tumor microenvironment; Scratch assay; Wound closure; Hyperglycemia; Macrophages; EMT; Type-2 diabetes; Cell migration

DOI:10.3390/ijms22105086

Hendricks A¹, Dall K¹, Brandt B², Geisen R³, Röder C⁴, Schafmayer C¹, Becker T¹, Hinz S¹, Sebens S⁴  

¹Department of General, Visceral, Thoracic, Transplantation and Pediatric Surgery, University Hospital Schleswig-Holstein Campus Kiel, Germany. 
²Institute of Clinical Chemistry, University Hospital Schleswig-Holstein Campus Kiel, Germany. 
³ORGA Labormanagement GmbH, Ochtrup, Germany. 
⁴Institute for Experimental Cancer Research, Kiel University and University Hospital Schleswig-Holstein Campus Kiel, Germany.

KEYWORDS:

Circulating tumor cells, Colorectal cancer, NYONE®cell imager, CK20 RT-qPCR, Longitudinal follow-up, Liquid biopsies

DOI:10.3389/fonc.2021.646885

Passman AM^1,2 et al.

¹School of Molecular Sciences, University of Western Australia, Crawley, WA 6009, Australia.

KEYWORDS:

CCL5 chemokine; Maraviroc; Hepatocellular carcinoma; Liver progenitor cells; macrophages; Hepatotoxicity; Cancer research

DOI:10.3390/cancers13194935

Flörkemeier I¹ et al.

¹Department of Gynecology and Obstetrics, Kiel University and University Medical Center Schleswig-Holstein Campus Kiel, Germany.

KEYWORDS:

OvCa; Chemotherapy; Drug development; Dual topoisomerase inhibitor; Apoptosis, 2D-imaging, 3D-imaging, co-culture; Primary cells; Hepatotoxicity; Cancer research

DOI:10.21203/rs.3.rs-743550/v1 

Fernandes S¹ et al.

¹LEPABE - Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, Portugal.

KEYWORDS:

Distribution network; Biofouling; Heat exchanger; Reynolds number; Shear stress; Stirrer tank; High shear stresses improve; Hydrodynamics 

DOI:10.1016/j.cej.2021.129348

Weber P¹ et al.

¹Experimental Pharmacology Mannheim (EPM), European Center for Angioscience (ECAS), Medical Faculty Mannheim, Heidelberg University, Germany.

KEYWORDS:

Adherens junction complex; Anchorage-dependent cell death; Endothelial cell; Non-canonical β-catenin signaling; Rho guanine nucleotide exchange factor; collagen I-coated
96-well culture plates; HUVEC; eGFP

DOI:10.3390/cells10040741

Ansari S^1,2, Mousavi A¹, Safarnejad MR², Farrokhi N³, Alavi SM ¹, Schillberg S⁴, Nölke G⁴

¹Department of Plant Molecular Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran
²Department of Plant Viruses, Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran
³Department of Cell & Molecular Biology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran
⁴Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Aachen, Germany

KEYWORDS:

Aflatoxin; Aspergillosis; Detection assay; Hybridoma technology; Immunofluorescence microscopy; Mannoproteins; Plant pathogens; Recombinant protein expression; Image analysis; mAb generation

DOI:10.1016/j.funbio.2021.03.004

Lokočová K¹ et al.

¹University of Chemistry and Technology, Department of Biotechnology, Prague, Czech Republic.

KEYWORDS:

Candida; Biofilm; Chitosan; Amphotericin B; Synergistic effect; C. albicans; C. parapsilosis: C. krusie; MTT assay; Image analysis

DOI:10.1134/S0026261721030061

Paudel BB^1,2,3 et al.

¹Department of Biochemistry & ²Quantitative Systems Biology Center (QSBC) , Vanderbilt University, Nashville, TN, USA.

³Department of Biomedical Engineering, University of Virginia, Charlottesville, VA, USA.

KEYWORDS:

Dose response; Drug combination assay; NRAS; NF1; BRAF; BRAFi; Melanoma cells; High-content imaging; Antioxidant capacity; Mathematical flux balance analyses; Redox; ROS; CellRox DeepRed

DOI:10.1158/0008-5472.CAN-19-3588 

Hayford C E¹ et al.

¹Chemical and Physical Biology Graduate Program, Vanderbilt University School of Medicine, Nashville, TN, USA.

KEYWORDS:

Tumor heterogeneity; Drug-response; Mutated oncogene; Targeted therapy response; Phenotypic states; Nuclei count 

DOI:10.1101/2020.06.05.136119

Zhang X^1,2 et al.

¹Institute for Developmental Cancer Therapeutics, West German Cancer Center, University Medicine Essen, Germany.

²Division of Solid Tumor Translational Oncology, German Cancer Consortium (DKTK, partner site University Hospital Essen) and German Cancer Research Center, DKFZ, Heidelberg, Germany.

KEYWORDS:

Pancreatic ductal adenocarcinoma (PDAC); BET inhibitor; HDAC inhibitor; Combined therapy; Dual BET/HDAC inhibitor; Proliferation assay

DOI:10.1002/ijc.33137

Gross T¹ et al.

¹Laboratory for MEMSApplications, IMTEK-Department of MicrosystemsEngineering, University of Freiburg, Georges-Koehler-Allee103, D-79110 Freiburg, Germany

KEYWORDS:

CRISPR/Cas9; f.sight; Cytena; Single-cell isolation; Gene editing; hTERT MSCs; Osteogenic differentiation; Transfection efficiency; High resolution; High-throughput; Fluorescent single cell cloning (FASCC); Colony growth monitoring; FACS; Limited dilution

DOI:10.1101/2020.08.17.253559

Walter F¹ et al.

¹ Institute for Experimental Cancer Research, Kiel University and University Medical Center Schleswig-Holstein (UKSH) Campus Kiel, Germany.

KEYWORDS:

Chitosan nanoparticles (CNPs) vehicle; NYONE^®; T cell response; Antigen-specific CD8⁺ T cells; Dendritic cells (DCs); Pancreatic ductal adenocarcinoma (PDAC) cells; Cell lysis; PI-staining; Cancer research

DOI:10.1371/journal.pone.0239369

Ohta H^1,2 et al.

¹Institute for the Advanced Study of Human Biology (ASHBi), Kyoto University, Japan.
²Department of Anatomy and Cell Biology, Graduate School of Medicine, Kyoto University, Japan.

KEYWORDS:

PGC-like cells (PGCLCs); Cyclosporin A; Epigenetic reprogramming; Fibroblast growth factor; in vitro expansion; Primordial germ cells (PGCs)

DOI:10.1093/biolre/ioaa195 

Josephides D¹, Hinchliffe M² et al.

¹Sphere Fluidics Ltd., Cambridge, UK.

²UCB, Slough, Berkshire, UK.

KEYWORDS:

High producer identification; CHO-cells; SCC; Brightfield & fluorescence; High-throughput screening (HTS); Microfluidics; Microtechnology; Monoclonality; Picodroplet; Colony outgrowth

DOI:10.1177/2472630319892571

Rauschenbach L^1,2,3,4 et al.

¹Institute of Reconstructive Neurobiology, Division of Stem Cell Pathologies;

²Life and Brain Centre, University of Bonn Medical Faculty and University Hospital Bonn, Germany; 

³DKFZ Division Translational Neurooncology at the West German Cancer Center (WTZ), DKTK Partner Site, University Hospital Essen, Germany; 

⁴Department of Neurosurgery and Spine Surgery, University Hospital Essen, Germany; German Cancer Consortium (DKTK), Germany.

KEYWORDS:

Autophagy; Glioblastoma; Repositioning; Ritonavir; Cancer; Temozolomide

DOI:10.1016/j.ejca.2020.09.017 

Gross T¹, Sinkala E², Zimmerman S¹

¹Laboratory for MEMS Applications, IMTEK-Department of Microsystems Engineering, University of Freiburg, Germany

²cytena GmbH, Neuer Messplatz 3, Freiburg, Germany

KEYWORDS:

CRISPR/Cas9; f.sight; Cytena; Single-cell isolation; Gene editing; Transfection efficiency; High-throughput; Fluorescent single cell cloning (FASCC); Colony growth monitoring

APPNOTE:www.cytena.com

Vernon M¹ et al.

¹Normandie Univ, UNICAEN, Inserm U1086 ANTICIPE (Interdisciplinary Research Unit for the Prevention and Treatment of Cancer), Biology and Innovative Therapies of Ovarian Cancers (BioTICLA), Caen, France.

KEYWORDS:

Ovarian tumor organiods; Chemoresistance; Cellular morphology; High-content imaging; High-throughput; Cytotoxic miRNAs 

DOI:10.1158/1535-7163.MCT-19-0510 

Giehring S¹

¹PAIA Biotech GmbH, Cologne, D-51105, Germany.

KEYWORDS:

PAIAplate; IgG; Cell line development (CLD); Fc and Fab glycosylation; High-throughput assays; Lectins; Monoclonal antibodies (mAB); Product quality; Titer assay; Bead-based assays; Bioprocess development

DOI:10.1007/978-1-0716-0191-4_15

Quilty F^1,2 et al.^3,4,5,6

¹School of Pharmacy and Pharmaceutical Sciences, Trinity Biomedical Sciences Institute, Trinity College Dublin, Ireland.

²School of Medicine, Trinity Translational Medicine Institute, Trinity College Dublin, Trinity Centre for Health Sciences, St James's Hospital, Ireland.

³Department of Histopathology and Morbid Anatomy, School of Medicine, Trinity College Dublin, Ireland.

⁴Cork Cancer Research Centre, BioSciences Institute, University College, Ireland.

⁵National Liver Institute, Menoufiya University, Egypt.

⁶Department of Gastroenterology, NIHR Nottingham Biomedical Research Centre, Nottingham University Hospitals NHS Trust and University of Nottingham, UK.

KEYWORDS:

Deoxycholic acid; Matrix metalloproteinases; mmp-10; Oesophageal adenocarcinoma; Wound healing; Scratch assay

DOI:10.3390/ijms21218042 

Munoz A¹ & Krasny A¹

¹nanocellect Biomedical Inc., 9525 Towne Centre Dr #150, San Diego, CA, USA. 

KEYWORDS:

HEK293T; Single-cell sorting; Gene editing; Transfection efficiency; GFP+; High-throughput; Fluorescent single cell cloning (SCC); Colony growth monitoring; High outgrowth

APPNOTE:www.nanocellect.com

Hendricks A¹, Brandt B², Geisen R³, Dall K¹, Röder C⁴, Schafmayer C¹, Becker T¹, Hinz S¹, Sebens S⁴

¹Department of General, Visceral, Thoracic, Transplantation and Pediatric Surgery, University Hospital Schleswig-Holstein Campus Kiel, Germany.

²Institute of Clinical Chemistry, University Hospital Schleswig-Holstein Campus Kiel, Germany.

³ORGA Labormanagement GmbH, Ochtrup, Germany.

⁴Institute for Experimental Cancer Research, Kiel University and University Hospital Schleswig-Holstein Campus Kiel, Germany.

KEYWORDS:

NYONE^®; ScreenCell^®; Circulating tumour cells; Colorectal cancer; Isolation by size of epithelial tumour cells; Liquid biopsy.

DOI:10.3390/cancers12092643

Strauss RP¹ et al.

¹School of Molecular Sciences, The University of Western AustraliaCrawley, WAAustralia.

KEYWORDS:

Liver progenitor cells (LPCs); ARF; INK4A; CDKN2A; Liver cancer; High-throughput imaging; Automated image analysis; Fluorescent proliferation assay

DOI:10.3727/105221620X15874935364268 

Kargaran PK¹, Kargaran PK¹, Evans JM², Bodbin SE³, Smith JGW⁴, Nelson TJ⁵, Denning C³, Mosqueira D³

¹Department of Cardiovascular Medicine, Center for Regenerative Medicine, Mayo Clinic, Rochester, MN, USA.

²Department of Health Science Research, Division of Biomedical Statistics and Informatics, Mayo Clinic, Rochester, MN, USA.

³Division of Cancer and Stem Cells, Biodiscovery Institute, University of Nottingham, Nottingham, UK.

⁴Faculty of Medicine and Health Sciences, Norwich Medical School, University of East Anglia, Norwich, UK.

⁵Division of General Internal Medicine, Division of Pediatric Cardiology, Departments of Medicine, Molecular Pharmacology, and Experimental Therapeutics, Mayo Clinic Center for Regenerative Medicine, Rochester, MN, USA.

KEYWORDS:

Hypertrophic cardiomyopathy (HCM); Disease modeling; Gene modifiers; High-content imaging; High-throughput; Haplogroups; Isogenic human pluripotent stem cell-derived cardiomyocytes; Mitochondrial DNA; Nuclei count

DOI:10.3390/jcm9082349 

Vaňková E¹ et al.

¹University of Chemistry and Technology, Department of Biotechnology, Prague, Czech Republic.

KEYWORDS:

Pterostilbene (PTE); Adhesion; Biofilm; Cell adhesion; Percentages of colonized area; Antibiofilm; Biofilm inhibition; Eradication; Gram-positive cocci; Pterostilbene

DOI:10.1007/s11274-020-02876-5 

Schöll-Naderer M¹, Helm O¹ , Spencker J² , Pfeifer L² , Rätsch T¹ , Sebens S¹, Classen B² 

¹Institute for Experimental Cancer Research, University of Kiel and University Hospital Schleswig-Holstein, Germany.

²Pharmaceutical Institute, Department of Pharmaceutical Biology, University of Kiel, Germany.

KEYWORDS:

Arabinogalactan; Galectin-3; Liver metastases; Pancreatic cancer; Pectin; Polysaccharide; Pancreatic ductal adenocarcinoma (PDAC); SiRNA knockdown; Automated wound closure monitoring; Scratch assay

DOI:10.1016/j.carres.2019.107903 

Bauer J¹ et al.

¹Boehringer Ingelheim Pharma GmbH & Co. KG, Early Stage Pharmaceutical Development, Pharmaceutical Development Biologicals, Biberach/Riss, Germany.

KEYWORDS:

In silico; CMC properties; Antibody engineering; Bioinformatic; Biotherapeutic development; Pharmaceutical industry; Monoclonal antibodies (mAbs); Microscopic investigation; Proof of monoclonality, CHO cells; Cell growth; High-throughput imaging; 384-well plate

DOI:10.1080/19420862.2020.1787121 

O'Brien NA¹, McDermott MSJ¹, Dylan Conklin¹, Luo T¹ , Raul Ayala¹ , Salgar S¹, Chau K¹, DiTomaso E^2,3, Babbar N², Su F  ², Gaither A^2,4, Hurvitz SA¹, Linnartz R², Rose K² , Samit Hirawat S^2,5, Slamon DJ^6,7 

¹Department of Medicine, Division of Hematology/Oncology, Geffen School of Medicine at UCLA, Los Angeles, CA, USA.

²Novartis Pharmaceuticals, Cambridge, MA, USA.

³Bayer Pharmaceuticals, Boston, MA, USA.

⁴LG Life Sciences, Cambridge, MA, USA.

⁵Bristol Myers Squibb, Lawrenceville, NJ, USA.

⁶Department of Medicine, Division of Hematology/Oncology, Geffen School of Medicine at UCLA, Los Angeles, CA, USA.

⁷UCLA Translational Oncology, Santa Monica, CA, USA.

KEYWORDS:

Breast cancer; Alpelisib; Palbociclib; Translational; CDK4/6; Activated PI3K/mTOR signaling; ER+/HER2-; High-throughput target screening; In vitro proliferation assays; Drug combination studies; IC₅₀; EFM19-PR cells

DOI:10.1186/s13058-020-01320-8 

Elanzew A^1,2, Nießing B³, Langendoerfer D², Rippel O^2,3, Piotrowski T³, Schenk F³, Kulik M³, Peitz M^1,4, Breitkreuz Y^1,2, Jung S³, Wanek P^5,6, Stappert L², Schmitt RH^3,7, Haupt S^1,2, Zenke M^5,6, König N³, Brüstle O^1,2

¹Institute of Reconstructive Neurobiology, University of Bonn Medical Faculty and University Hospital Bonn, Germany.

²LIFE&BRAIN GmbH, Cellomics Unit, Bonn, Germany.

³Fraunhofer Institute for Production Technology, Aachen, Germany.

⁴Cell Programming Core Facility, University of Bonn Medical Faculty, Bonn, Germany.

⁵Institute for Biomedical Engineering, Cell Biology, Faculty of Medicine, RWTH Aachen University, Germany.

⁶Helmholtz Institute for Biomedical Engineering, RWTH Aachen University, Germany.

⁷Laboratory for Machine Tools and Production, RWTH Aachen University, Aachen, Germany.

KEYWORDS:

Automation; Cell culture; Cell production; Induced pluripotent stem cells; iPS; Reprogramming; Confluence 

DOI:10.3389/fbioe.2020.580352 

Bodbin SE¹, Denning C¹ & Mosqueira D¹

¹Division of Cancer and Stem Cells, Biodiscovery Institute, University of Nottingham, Nottingham, UK.

KEYWORDS:

Disease modelling; Human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs); Transfection efficiency; Viafect^TM; High content imaging system

DOI:10.3390/mps3030057 

Jordan I¹, Horn D², Thiele K^2,3  et al.

¹⁺²ProBioGen AG, Herbert-Bayer-Straße 8, 13086, Berlin, Germany.

³Sartorius Stedim Cellca GmbH, Erwin-Rentschler-Str 21, 88471, Laupheim, Germany.

KEYWORDS:

Modified vaccinia Ankara (MVA); MVA-CR19; CR.pIX; Suspension cell line; Vaccine production; Genome stability

DOI:10.1007/s12250-019-00176-3

Czechowska K et al.¹

¹Altmattstrasse 1B, 6418 Rothenthurm, Switzerland.

Participating institutions e.g.: 

Center for Biologics Evaluation and Research, Food and Drug Administration (FDA), Silver Spring, MD;  GlaxoSmithKline, Stevenage, UK;  Terry Fox Laboratory-BC Cancer, Canada;  Novartis Pharma AG, Basel, Switzerland;  Schwiete-Laboratory Microbiota and Inflammation, German Rheumatism Research Centre Berlin (DRFZ), a Leibniz Institute, Germany;  MedImmune, an AstraZeneca Company, Gaithersburg, MD;  Biogen, Acute Neurology, Cambridge, MA;  Novo Nordisk Center for Stem Cell Biology; Copenhagen, Denmark; Cardiff University, UK;  Memorial Sloan Kettering Cancer Center, NY;  Genentech, San Francisco, CA;  Bristol-Myers Squibb, Princeton, NJ;  University of Turku and Åbo Akademi University, Finland;  Max Planck Institute for Biology of Ageing, Germany;  Eurofins Pharma Bioanalysis Services UK Ltd, UK;  University of South Alabama, Mobile, AL;  BD Biosciences, California; University of Cambridge, UK;  Helmholtz Center for Environmental Research (UFZ), Leipzig, Germany;  King's College London, UK;  The University of Tokyo;  Dana-Faber Cancer Institute, Boston, MA;  National Institute of Health (NIH), MD;  Weizmann Institute of Science, Israel;  Fred Hutchinson Cancer Research Center, Seattle, WA;  TTP plc, UK;  TU München, Germany;  Amerimmune, Fairfax, VA; University of São Paulo, Brazil;  VIB Center for Inflammation Research, Belgium; The University of Sydney and Centenary Institute, Australia;  Harvard Medical School, Boston, MA;  Roswell Park Comprehensive Cancer Center, NY;  Fraunhofer Institute for Cell Therapy and Immunology IZI, Leipzig, Germany.

KEYWORDS:

Cytometry; Quality Assurance; Reproducibility; FDA; EMA; NIST; Assurance of Clonality; Single Cells; Monoclonality

DOI:10.1002/cyto.a.23777

Paudel BB^1,2 et al.

¹Department of Biochemistry & ²Quantitative Systems Biology Center (QSBC) , Vanderbilt University, Nashville, TN, USA.

KEYWORDS:

Fluorescence microscopy; Systems biology, Redox capacity, Reactive oxygen species (ROS); CellRox TM DeepRed; Drug combination assay; High-content imaging

DOI:10.1101/818989

Vochozkova P¹ et al.

¹Institute for Molecular Animal Breeding and Biotechnology, LMU Munich, Munich, Germany.

KEYWORDS:

CRISPR/Cas; Primary cells; Single cell clones; gRNA

DOI:10.1007/978-1-4939-9170-9_17

Mosqueira D¹ et al.

¹Department of Stem Cell Biology, Centre of Biomolecular Sciences, University of Nottingham, Nottingham NG7 2RD, UK.

KEYWORDS:

Cardiomyocytes; Cellular models; Disease modelling; Drug screening; High-content imaging; High-throughput; Hypertrophic cardiomyopathy; Hypertrophy; Mitochondrial respiration; Phenotyping; Mitochondria

DOI:10.3390/mps2040083

Kleemann M^1,2 et al.

¹Institute of Applied Biotechnology, University of Applied Sciences Biberach, Germany.

²Faculty of Medicine, University of Ulm, Germany.

KEYWORDS:

Ovarian cancer; MicroRNA; RAF1; Apoptosis; Signalling pathways; Targets; High-throughput; Confluence

DOI: 10.1007/s00018-018-2958-x

Santos GL¹ et al.

¹Institute of Pharmacology and Toxicology, University Medical Center Göttingen, Germany; Randall Centre for Cell and Molecular Biophysics, King's College London, London, UK; DZHK (German Center for Cardiovascular Research) Partner Site, Göttingen, Germany.

KEYWORDS:

Cardiac fibrosis; Myofibroblasts; Neonatal rat cardiac fibroblasts (NRCF)Proliferation assay over time; Hoechst; FITC-phalloidin; High-content imaging

DOI:10.1016/j.yjmcc.2019.06.015

McKenna MT^1,2 et al.

¹Vanderbilt University Institute of Imaging Science, Vanderbilt University, Nashville, TN, United States.

KEYWORDS:

Breast cancer; Doxorubicin; Mathematical modeling; Pharmacodynamics; Pharmacokinetic modeling; Treatment response; Dose response; Fluorescence; High-content imaging

DOI:10.3389/fphys.2019.00616

Chen C¹ et al.

¹Amgen Inc., Drug Substance Technologies, Process Development, CA, USA.

KEYWORDS:

Cell line development (CLD); Clonality; Cell imaging; Limiting dilution; Probability of clonality; Single cell cloning (SCC)

DOI:10.1002/biot.201900289

Schubert M¹ et al.

¹Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Department of Plant Biotechnology, Aachen, Germany.

KEYWORDS:

Aspergillus antigen; Detection assay; Epitope identification; Galactofuranose; Glycobiology; Limited dilution; B-lymphocytes fused to myelomaHybridoma cells; Antibody generation; IgG; 

DOI:10.3389/fcimb.2019.00234

Coronel J¹, Heinrich C², Klausing S², Noll T³, Figueredo-Cardero A⁴, Castilho LR¹.

¹Federal University of Rio de Janeiro (UFRJ), COPPE, Cell Culture Engineering Laboratory, Rio de Janeiro, RJ, Brazil.

²Xell AG, Alte Verler Straße 1, Bielefeld, Germany.

³Bielefeld University, Institute of Cell Culture Technology, Universitätsstraße 25, Bielefeld, Germany.

⁴Fiocruz, Bio-Manguinhos, Rio de Janeiro, RJ, Brazil.

KEYWORDS:

CHO perfusion cultivation; Mild hypothermia; Productivity; Recombinant factor VIII; Valeric acid; Single cell cloning (SCC)

DOI:10.1002/btpr.2915

Fieder J¹ et al.

¹Boehringer Ingelheim GmbH & Co. KG, Early Stage Bioprocess Development, Biberach, Germany.

KEYWORDS:

Biopharmaceutical production cell line; Assurance of clonality; FACS; Imaging; Single cell cloning; SCC; Calcein; FASCC

DOI:10.1002/biot.201700002

Bittner M¹ et al.

¹University of Chemistry and Technology, Department of Biotechnology, Prague, Czech Republic.

KEYWORDS:

Megasphaera sp; Cell adhesion; Solid surfaces; Surface interaction; XDLVO theory; Brewery

DOI:10.1002/jib.415

Makita Y¹ et al.

¹Takeda Pharmaceutical Company Limited, Pharmaceutical Research Division, Fujisawa, Kanagawa 251-8555, Japan.

KEYWORDS:

Erlotinib; Kinetochore-associated Protein 2; Lipid Nanoparticle; Lung Cancer; Patient-derived Tumor Xenograft; Short interfering RNA; siRNA; Three-dimensional Culture; 3D

DOI:10.3892/ol.2018.7890

Loebrich S¹ et al.

¹ImmunoGen, Cell Line and Upstream Process Development & Technical Operations, Waltham , USA.

KEYWORDS:

ADC; DoE; Feed additives; Fed-batch; Media development; Multivariate analysis; Product quality; Scalability; Monoclonal antibodies (mAB); Glycosylation; Trypan blue; 24-w deepwell plates

DOI:10.1080/19420862.2018.1527665

Mora A^1,2 et al.

¹AbbVie Bioresearch Center, Process Sciences Department, Worcester, MA, USA.

²Francis College of Engineering, University of Massachusetts Lowell, Lowell, MA, USA.

KEYWORDS:

Cell line development; Chinese hamster ovary (CHO); Design of experiment; Feed medium; Multivariate data analysis; Viability; Trypan blue; Fed-batch; 24-deep well plates

DOI:10.1007/s10616-019-00350-1

Wang B¹ et al.

¹MedImmune, Cell Culture and Fermentation Sciences, Biopharmaceutical Development, Gaithersburg, MD, USA

KEYWORDS:

Automation; Cell imaging; Cell line development (CLD); Hamilton/96DW process; Single-cell cloning (SCC); Monoclonality; mAB; Protein production; High-throughput screening; Trypan blue

DOI:10.1002/btpr.2721

Ashraf Abdelkhaliq¹ et al.

¹RIKILT-Wageningen Research, P.O. Box 230, 6700 AE Wageningen, The Netherlands

KEYWORDS: Nanoparticles; High throughput screening; Cellular adhesion and uptake; Label-free LC–MS/MS; Quantitative proteomics

DOI:10.1186/s12951-018-0394-6

Handl A ¹ et al.

¹Institute of Applied Biotechnology, Biberach University of Applied Sciences, Biberach, Germany.

KEYWORDS:

Fluorescence microscopy; Fluorescent dyes; Monoclonal antibody (mAB); IgG; Protein aggregation; Bis-ANS; High-content imaging

DOI:10.1039/c9an00424f

Knaack H¹ et al.

¹Institute for Experimental Cancer Research, Christian-Albrechts-University Kiel (CAU) and UKSH Campus Kiel, Germany.

KEYWORDS:

Cancer stem cell; Epithelial-mesenchymal-transition; Hepatic microenvironment; Pancreatic ductal adenocarcinoma; Non-invasive cell confluence; Single cell cloning; Clone expansion

DOI:10.18632/oncotarget.25884

Matsui TK¹, Mori E².

¹Department of Future Basic Medicine & Department of Neurology, Nara Medical University, Japan.

²Department of Future Basic Medicine, Nara Medical University, Japan.

KEYWORDS:

Brain development; Central nervous system; Microglia; Neural stem/progenitor cell

DOI:10.1016/j.bbrc.2018.07.130

Kleemann M^1,2 et al.

¹Institute of Applied Biotechnology, University of Applied Sciences Biberach, Germany.

²Faculty of Medicine, University of Ulm, Germany.

KEYWORDS:

Cisplatin resistant; MicroRNA (miRNA); Apoptosis; Signaling pathway; Intrinsic apoptotic pathway; Non-invasive cell confluence; Cell death; Ovarian cancer; Target analysis; miR-217-5p; Adherent cell culture; SKOV3; OVCAR3; siRNA death control (DT); Etoposide; Paclitaxel

DOI:10.1038/s41598-018-27438-6

Kolouchová I¹ et al.

¹University of Chemistry and Technology, Department of Biotechnology, Prague, Czech Republic.

KEYWORDS:

Adhesion; Baicalein; Biofilm; Eradication; Pterostilbene; Resveratrol; Cell adhesion; Solid surfaces; Surface interaction 

DOI:10.1007/s12223-017-0549-0

Diederichs T¹ et al.

¹Institute of Biochemistry, Biocenter, Goethe University Frankfurt, Germany.

KEYWORDS:

Silicon-on-insulator chips; Membrane proteins; Microcavities; Nanopores; Optical readout; Supported lipid bilayers; Transport kinetics; Membrane-on-a-chip; Membrane transport; Microstructured Si/SiO2 chip

DOI:10.1021/acs.nanolett.8b01252

McKenna MT^1,2 et al.

¹Vanderbilt University, Institute of Imaging Science & ²Department of Biomedical Engineering, Nashville, TN, United States.

KEYWORDS:

Mathematical modeling; Oncology; Pharmacology; Pharmacodynamics; Pharmacokinetic modeling; Treatment response; Dose response; Fluorescence; High-content imaging; Nuclei; Cell-population over time

DOI:10.1007/s10439-018-2001-2

Mathias S¹ et al.

¹University of Applied Sciences Biberach, Institute of Applied Biotechnology, Germany. 

KEYWORDS:

Bispecific antibody production; CHO cell line engineering; Difficult-to-express proteins; Production bottleneck; Secretory pathway; Fed-batch; Immunocytochemistry; High-content analysis; Evaluation of the intracellular distribution of the synthesised IgG

DOI:10.1016/j.jbiotec.2018.02.009

Ohta H¹ et al.

¹Department of Anatomy and Cell Biology, Graduate School of Medicine, Kyoto University, Yoshida-Konoe-cho, Sakyo-ku, Kyoto, Japan

KEYWORDS:

CiRA; Imaging cells on feeder layer; PGCLC proliferation; Embryonic stem cells (ESCs); Forskolin; Chemical library screening

DOI:10.15252/embj.201695862

Mathur V¹ et al.

¹Department of Neurology and Neurological Sciences, School of Medicine, Stanford University, CA, USA.

KEYWORDS:

STING; Experimental autoimmune encephalomyelitis; Ganciclovir; Microglia; Neuroinflammation; Type I interferon response; Toxicity; Celltox Green; Cell confluence

DOI:10.1016/j.neuron.2017.11.032

McKenna MT ¹ et al.

¹Vanderbilt University Institute of Imaging Science & Department of Biomedical Engineering, Nashville, USA.

KEYWORDS:

Breast cancer; Doxorubicin; Treatment response studies; Fluorescence microscopy; 20x Lens; Predictive Mathematical Modeling Approach

DOI:10.1038/s41598-017-05902-z

Fieder J¹ et al.

¹Boehringer Ingelheim GmbH & Co. KG, Early Stage Bioprocess Development, Boehringer Ingelheim GmbH & Co. KG, Biberach, Germany.

KEYWORDS:

Biopharmaceutical production cell line; Clonality; FACS; Imaging; SCC; FASCC; CLD

DOI:10.1002/biot.201700002

Miyauchi H¹ et al.

¹Department of Anatomy and Cell Biology, Graduate School of Medicine, Kyoto University, Kyoto, Japan.

KEYWORDS:

CiRA; Bone morphogenetic protein (BMP); Female germ‐cell fate; Meiosis; Primordial germ cell‐like cells; Retinoic acid; GFP; Alexa Fluor 633; (PGCs)/PGC-like cells (PGCLCs); Embryonic stem cells (ESCs)

DOI:10.15252/embj.201796875

Lundstrom K¹

¹PanTherapeutics, Lutry, Switzerland.

KEYWORDS:

Label-free; Cell-impedance-based; Screening assays; Dose-response; High-content; IC50; EC50

DOI:10.1080/17460441.2017.1297419

Hardeman KN¹ et al.

¹Department of Cancer Biology, Vanderbilt University School of Medicine, Nashville, TN 37232, USA.

KEYWORDS:

Melanoma; Skin cancer; Drug treatment; Proliferation; Dose response; Glycolysis Sensitizes BRAF-mutated Melanomas; BRAF inhibition

DOI:10.1038/srep42604

Lu J¹ et al.

¹BioMarin Pharmaceutical Inc., Cell Culture Process Development, Novato, CA 94949, USA.

KEYWORDS:

Viability; Trypan blue; NYONE identical cell growth ranking as Vi-Cell XR; Deep well plates (DWP); CLD; AMBR15; Freedom EVO liquid handler system; BioHT metabolic analyzer

DOI:10.1186/s12919-018-0097-x

Camire J¹ et al.

¹GE Healthcare, Logan, UT, USA.

KEYWORDS:

Antioxidants; Chinese hamster ovary (CHO) cell; Process optimization; Recombinant protein production; Small molecule enhancers; Trypan blue; Apoptosis

DOI:10.1007/s00449-017-1767-1

Markert S¹ et al.

¹Roche Diagnostics GmbH-Pharmaceutical Biotech Production and Development, Nonnenwald 2, 82377 Penzberg, Germany.

KEYWORDS:

Biopharmaceutical drug development; Fed-batch CHO suspension cultures; High-throughput process optimization; Industrial cell culture automation; Scale up prediction; Shaken microtiter plate

DOI:10.1002/bit.26044

Hanker AB¹ et al.

¹Department of Medicine & Breast Cancer Program, Vanderbilt-Ingram Cancer Center, Vanderbilt University Medical Center, Nashville, TN, USA.

KEYWORDS:

Breast cancer; HER2⁺; Trastuzumab; Pertuzumab; Cell Viability Imaging Kit; Proliferation

DOI:10.1158/0008-5472.CAN-16-2808

Paul AJ¹ et al.

¹Sartorius Stedim Cellca GmbH, 88471 Laupheim, Baden-Württemberg, Germany.

KEYWORDS:

Biopharmaceutical production cell line; Clonality; FACS; Imaging; Single cell cloning (SCC); CLD; IgG; Viability; Cell aggregation levels 

DOI:10.1186/s12919-018-0097-x

Paul AJ¹ et al.

¹Institute of Applied Biotechnology, Biberach University of Applied Sciences, Biberach, Germany.

KEYWORDS:

Automatization; Fluorescence microscopy; Fluorescent dyes; Monoclonal antibody; Protein aggregation; Bis-ANS; High-content imaging

DOI:10.1007/s00216-017-0362-2

Giehring S¹ et al.

¹PAIA Biotech GmbH, Cologne, D-51105, Germany.

KEYWORDS:

PAIAplate; IgG; Bead-based assays; Bioprocess development; Cell line development (CLD); Fc and Fab glycosylation; High-throughput assays; Lectins; Monoclonal antibodies; Product quality; Titer assay; mAB

DOI:10.1186/s12919-018-0097-x

Paul AJ¹ et al.

¹Institute of Applied Biotechnology, Biberach University of Applied Sciences, Biberach, Germany.

KEYWORDS:

CHO cells; Bioprocess engineering; Monoclonal antibodies (mAB); Protein aggregation; Automatization; Fluorescence microscopy; Bis-ANS; High-content imaging

DOI:10.1002/bit.26534

Wright C¹ et al.

¹Biogen, Technical Development, Cambridge, MA.

KEYWORDS:

CHO; Cell line engineering; Early cell line development; Product quality; CLD; Monoclonality; High-throughput imaging

DOI:10.1007/s12079-017-0410-x

Flum M^1,2 et al.

¹Institute of Applied Biotechnology, University of Applied Sciences Biberach, Germany.

²Faculty of Medicine, University of Ulm, Germany.

KEYWORDS:

MicroRNA (miRNA); Apoptosis; siRNA death control (DT); Non-invasive cell confluence; Cell death; Colorectal cancer; Target analysis; miR-217-5p; Adherent cell culture; miRNA mimic transfection

DOI:10.1007/s12079-017-0410-x

Fischer S^1,2 et al.

¹Boehringer Ingelheim GmbH & Co. KG, Early Stage Bioprocess Development, Biberach, Germany.

²Boehringer Ingelheim GmbH & Co. KG, Cell Culture Development CMB, Biberach, Germany.

KEYWORDS:

Chinese hamster ovary (CHO) cells; Cell engineering; Difficult-to-express proteins; miR-557; microRNA; Monoclonal antibody (mAB); Cell line development (CLD); SCC; Monoclonality

DOI:10.1002/bit.26280

de Almeida TS¹ et al.

¹Department of Biochemistry and Molecular Biology, Federal University of Ceará, 60020-181, Fortaleza, CE, Brazil.

KEYWORDS:

Cell imaging; DPPH; MCF-7 cells; Medicinal plant; TBARS; CellROX Deep Red; SC50; IC50; ROS; Hoechst; Nuclear counterstain

DOI:10.1016/j.biopha.2017.07.050

Matatkova O¹ et al.

¹ Biotechnology, University of Chemistry and Technology, Department of Biotechnology, Prague, Czech Republic.

KEYWORDS:

Biofilm; Candida sp.; Trichosporon sp.; Rhamnolipid; Amphotericin B; Drugs interaction 

DOI:10.1007/s11696-017-0141-8

Bittner M¹ et al.

¹University of Chemistry and Technology, Department of Biotechnology, Prague, Czech Republic.

KEYWORDS:

Megasphaera sp; Cell adhesion; Solid surfaces; Surface interaction; XDLVO theory; Brewery

DOI:10.1002/jib.415

Reinartz R^1,2 et al.

¹Stem Cell Pathologies, University of Bonn Medical Center, Bonn, Germany.

²Institute of Reconstructive Neurobiology, University of Bonn Medical Center, Bonn, Germany.

KEYWORDS:

Human glioblastoma; Derivation of tumor subclones; Cell morphology measurement; Compound screening; Drug–response profiles; IC50

DOI:10.1158/1078-0432.CCR-15-2089

Wright C¹ et al.

¹Biogen, Cell Culture Development, Cambridge, MA, USA.

KEYWORDS:

CHO cells; Amplicon sequencing; Early stage cell line development (CLD); Mutation detection; FASCC; Monoclonal cell lines; Clonality

DOI:10.1002/btpr.2263

Meyer HJ¹ et al.

¹Genentech, Dept. of Early Stage Cell Culture & Dept. of Discovery Oncology, South San Francisco, CA, USA.

KEYWORDS:

HEK293; Antibody expression; miRNA; Protein production; Transient transfection; Valproic acid; Cell viability

DOI:10.1002/btpr.2488

Woo K¹ et al.

¹The Centre for Medical Research, The Perkins Institute of Medical Research, Nedlands, WA 6009, Australia.

KEYWORDS:

Analogues; Apoptosis; Lenalidomide; Liver cancer; Mitosis; Progenitor cells; Sorafenib; Thalidomide

DOI:10.1016/j.ejmech.2016.03.015

Urban M¹ et al.

¹Institute of Biochemistry, Biocenter, Goethe University Frankfurt, Germany.

KEYWORDS:

Model lipid bilayers; Pore-spanning membrane; Nanopores; Suspended bilayer; Lab-on-chip, Biosensor; Membrane proteins; Microcavities; Nanopores; Optical readout; Supported lipid bilayers; Transport kinetics; Membrane-on-a-chip; Microstructured Si/SiO2 chip

DOI:10.3791/53373

Jaeger PA^1,2,3 et al.

¹Department of Neurology and Neurological Sciences, Stanford University School of Medicine, CA, USA. pjaeger@ucsd.edu.

²Institute of Chemistry and Biochemistry, Free University Berlin, Berlin, Germany.

³Departments of Bioengineering and Medicine, University of California San Diego, La Jolla, CA, USA.

KEYWORDS:

Growth-differentiation factor (GDF); Alzheimer’s disease (AD); Bone morphogenetic protein (BMP); Bromodeoxyuridine (BrdU); Neural progenitor cells (NPC); eGFP; Proliferation

DOI:10.1186/s13024-016-0095-2

Kirby ED¹ et al.

¹Stanford University, Department of Neurology and Neurological Sciences and Center for Tissue Regeneration, Repair, and Restoration, CA, USA

KEYWORDS:

Neural stem and progenitor cells (NSPCs); Immunostaining; Immunocytochemistry (ICC); Adult neurogenesis; Hippocampus; Neural precursor; Stem cell; Vascular endothelial growth factor (VEGF)

DOI:10.1073/pnas.1422448112

Evans K¹ et al.

¹MedImmune, Cell Culture and Fermentation Sciences, Biopharmaceutical Development, Gaithersburg, MD, USA

KEYWORDS:

Cell imaging; Cell line development (CLD); Flow cytometry; Mammalian cell culture; Single-cell cloning (SCC); Monoclonality; mAB; Protein production; Limited dilution; Single cell deposition; International Conference on Harmonisation of Technical
Requirements for Registration of Pharmaceuticals for Human Use (ICH); United States Food and Drug Administration (FDA); CHO suspension cell line

DOI:10.1002/btpr.2145

Lehmann R¹ et al.

¹Center for Life Science Automation (celisca), University of Rostock, Germany.

KEYWORDS:

Biomek Cell Workstation; Automated cell culturing; Life science automation; Suspension cells; High-content screening (HCS); Liquid handling

DOI:10.1177/2211068215599786

Moritz B¹, Becker PB², Göpfert U¹.

¹Roche Pharmaceutical Research and Early Development, Large Molecule Research, Penzberg, Germany.

²Biomedical Center and Center for Integrated Protein Science Munich, Ludwig Maximilian University, Munich, Germany.

KEYWORDS:

Transfection ; Human cytomegalovirus (hCMV-MIE); CHO cells; Recombinant gene expression; Production of therapeutic antibodies; CLD; Cell density; Viability of mammalian cell culture

DOI:10.1038/srep16952

Kvasnickova E¹ et al.

¹Department of Biotechnology, University of Chemistry and Technology Prague, Czech Republic.

KEYWORDS:

Baicalein; Antifungal/anti-biofilm activity; Candida krusei; Candida parapsilosis; Chitosan; Eradication; Antimicrobial agents; Inhibition; 96-well format

DOI:10.1016/j.mimet.2015.09.002

Byrne AM¹ et al.

¹Department of Clinical Medicine, Institute of Molecular Medicine, Trinity College, Dublin, Ireland.

KEYWORDS:

Bile acids; Golgi; Barrett’s oesophagus; Oesophageal cancer; GOLPH2; Adenocarcinoma; siRNA; Scratch wound-healing assay; Wound closure %

DOI:10.1038/bjc.2015.350

Paul AJ¹ and Hesse F¹

¹Institute of Applied Biotechnology (IAB), Biberach University of Applied Sciences, Biberach, Germany.

KEYWORDS:

Product quality; mAb aggregates; CHO DG44 cells; Bis-ANS; Protein aggregation

DOI:10.1186/1753-6561-9-S9-O4

Wirtz ED¹, Hoshino D², Maldonado AT³, Tyson DR³, Weaver AM³.

¹Department of Otolaryngology-Head and Neck Surgery, Tripler Army Medical Center, Honolulu, Hawaii.

²Kanagawa Cancer Center, Division of Cancer Cell Research, Yokohama, Kanagawa, Japan.

³Department of Cancer Biology, Vanderbilt University Medical Center, Nashville, Tennessee.

KEYWORDS:

Cancer; Tumor; Head and Neck squamous cell carcinoma (HNSCC); PIK3CA mutation; IC50; Oncogenes; Cell Viability;
Calcein; Hoechst; Propidium iodide

DOI:10.1001/jamaoto.2015.0471

Kameoka S¹, Babiarz J¹, Kolaja K¹, Chiao E²

¹Hoffmann-La Roche, Early and Investigative Safety, Nonclinical Safety, New Jersey, USA

²Biogen Idec, 14 Cambridge Center, Bld., Cambridge, USA

KEYWORDS:

Human cell-based assays; Human pluripotent stem cell test (hPST); Preclinical toxicology teratogenicity studies; Environmental toxicants; High-throughput screening (HTS)

DOI:10.1093/toxsci/kft239

Ding Z¹ et al.

¹Stanford University School of Medicine, Department of Radiology; Stanford, CA; USA

KEYWORDS:

Central nervous system (CNS); experimental autoimmune encephalomyelitis (EAE); Ganciclovir (GCV); Glial
fibrillary acidic protein (GFAP); Multiple sclerosis (MS); Neuroinflammation; Neurodegenerative diseases; Microglia

DOI:10.1084/jem.20120696

Bos AB¹ et al.

¹Department of Early Stage Cell Culture, Genentech Inc., South San Francisco, USA.

KEYWORDS:

Transient transfection; High throughput (HT); Tubespins; Automation; Protein expression systems; HEK293T; Proof of concept, Antibody development; Biological structure and functional analysis; human IgG1 (hu IgG1)

DOI:10.1016/j.jbiotec.2014.03.027

Brännmark C¹ et al.

¹Reagent and Assay Development Discovery Sciences R&D, Astra Zeneca, Mölndal, Sweden.

KEYWORDS:

Obesity; Type 2 diabetes; Human adipose derived stem cells; differentiated adipocytes; in vitro; PCL fiber plates (Nanofiber Solutions)

DOI:10.1371/journal.pone.0113620

Kusters I¹ et al.

¹Department of Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute and the Zernike Institute for Advanced Materials, University of Groningen, The Netherlands.

KEYWORDS:

Membrane-on-a-chip; GUV; HTS; Membrane transport; microstructured Si/SiO2 chip; Transport kinetics; Single-membrane channels; Micropore array; Suspended lipid bilayers; Virus fusion; Hemolysin

DOI:10.1021/nn405884a

Wieland A¹ et al.

¹Stem Cell Pathologies, Institute of Medical Biometry, Informatics and Epidemiology, University of Bonn Medical Center, Bonn, Germany.

KEYWORDS:

Primary human Glioblastoma; Brain tumor; Cancer research; Niclosamide; Clinical development; Cytotoxicity; Dose response; Cell-cycle progression; Apoptosis; Cell migration & Proliferation; Co-culture experiments; Lentiviral transduction

DOI:10.1158/1078-0432.CCR-12-2895

Udyavar AR¹ et al.

¹Department of Cancer Biology, Vanderbilt University, Nashville, USA.

KEYWORDS:

Spleen Tyrosine Kinase (SYK); Biomarker; Potential therapeutic target; Small-cell lung cancer (SCLC); Live/Dead assay; Viability

DOI:10.1186/1752-0509-7-S5-S1

Gonzalez R¹ et al.

¹International Stem Cell Corporation, 5950 Priestly Drive, Carlsbad, CA 92008, USA.

KEYWORDS:

Neural stem cells (NSCs); Parkinson’s disease (PD); Substantia nigra (SN); Human pluripotent stem cells (hPSCs); Blood brain barrier; NB medium (NeuroBasal medium); Measuring neurite density

DOI:10.1001/jamaoto.2015.0471

Kolesinska B¹ et al.

¹Laboratorium für Organische Chemie, Departement Chemie und Angewandte Biowissenschaften, ETH-Zürich, Switzerland.

KEYWORDS:

Membrane-on-a-chip; nanoFAST Biochip; GUV; HTS; Membrane transport; Microstructured Si/SiO2 chip; Micropore array; Transport kinetics; Single-membrane channels; Suspended lipid bilayers; Proline; Inverted Microscopy; High-content 20x

DOI:10.1002/cbdv.201200393

Vaes B¹ et al.

¹ReGenesys BVBA Heverlee, Belgium.

KEYWORDS:

GvHD prophylaxis; MultiStem cells; Adherent stem cells; Bioreactor; Regenerative medicine; Cell morphology measurement

DOI:10.3389/fimmu.2012.00345

Mosher KI¹ et al.

¹Department of Neurology and Neurological Sciences, Stanford University School of Medicine, Stanford, California, USA.

KEYWORDS:

Neural progenitor cells (NPCs); Microglia proliferation; CNS trauma or disease; Chemotaxis assay; Filter assay; Transwell assay; Hoechst 33342

DOI:10.1038/nn.3233

Franscini N¹ et al.

¹Institute of Chemistry and Biological Chemistry, Zurich University of Applied Sciences, Wädenswil, Switzerland.

KEYWORDS:

Automated cellculture platform; Liquid handling; Human primary intervertebral disc cells; Cell isolation; Cell expansion; Cell characterization; RoboFlasks; Glass chamber slides

DOI:10.1016/j.jala.2011.01.002

Hiramatsu K¹ et al.

¹Department of Bone and Cartilage Biology, Osaka University Graduate School of Medicine, Suita, Osaka, Japan.

KEYWORDS:

iPS cells; Induced pluripotent stem cells; Polygonal chondrogenic cells; Cartilage injury; Hyaline cartilage; Chondrocytes; Chondrogenic factor; SOX9; CiRA; Anti Collagen immuno-staining

DOI:10.1172/JCI44605

Jain S¹ et al.

¹VUMC-Clinical Genetics, Amsterdam, the Netherlands.

KEYWORDS:

Automation; Robotics; Cell-based assays; CNS and PNS diseases; Genomics; High-content screening; HCS; High-throughput; HT; Liquid handling

DOI:10.1177/1087057111413920