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Imaging Systems and Automation Solutions

Our customers are supported by supplying over 30 years of experience of the founders. By engineering and providing fluorescence and brightfield imaging systems and applications, SYNENTEC introduces the most versatile cell imaging systems on the market, as well as offering proprietary automation solutions such as plate handlers and further more ready-to-use assay kits.

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Automated Imaging – Technology Makes The Difference

SYNENTEC’s fully automated high throughput cell imaging systems are designed for the characterization of cells as a significant step of your scientific research, development and production process.

Using image cytometry with CELLAVISTA® 4 and NYONE® many labor intensive processes can be optimized: images from a large number of samples are automatically converted into unbiased numbers for cell culture status with our YT‑software®, which is constantly being expanded and which contains data batch-processing solutions for highest claims in plate throughput and automated data handling.

We know that time is an increasingly scare resource these days. Therefore we developed also automated high throughput SYBOT-800® plate handler as well as ready-to-use Assay-Kits for complete one-hand solutions!

Features

  • Characterization of Adherent and Suspension Cells, Microorganisms, Tissue Sections, Model Organisms
  • Standard Format Micro Plates (6-384), T-flasks, Culture Dishes, Microscope Slides
  • Low Sample- and Reagent Consumption
  • Automated highly precise and fast Laser based Autofocus
  • Non-invasive, sterile White Light Imaging (transparent lid is mandatory)
  • Highly sensitive Fluorescence Detection
  • Automatic Lens-Changer
  • Motor driven free combinable Emission and Excitation Filters
  • Embedded ultra fast Image Analysis with more than 30 different applications

Compare Technical Specifications

CELLAVISTA® 4 HighEnd1 NYONE® HighEnd1 CELLAVISTA® 4 Scientific NYONE® Scientific
Throughput 2502 plates / day 1002 plates / day 2502 plates / day 1502 plates / day
Objective capacity 4 3 4 3
Selectable Resolutions1 3.3 µm @ 2x optional
2 µm @ 4x
1.1 µm @ 10x
0.53 µm @ 20x
0.35 µm @ 40x
2 µm @ 4x
1.1 µm @ 10x
0.53 µm @ 20x
6.5 µm @ 2x optional
3.25 µm @ 4x
1.3 µm @ 10x
0.65 µm @ 20x
0.33 µm @ 40x
6.5 µm @ 2x optional
3.25 µm @ 4x
1.3 µm @ 10x
0.65 µm @ 20x
0.33 µm @ 40x optional
Automated whole well/whole plate imaging
Sample types SBS plate format (6-384 wells), culture dishes and microscope slides SBS plate format (6-384 wells), culture dishes and microscope slides SBS plate format (6-384 wells), culture dishes and microscope slides SBS plate format (6-384 wells), culture dishes and microscope slides
Camera 8 bit progressive scan
CMOS
8 bit progressive scan CMOS 16 bit sCMOS 16 bit sCMOS
Pixel density 3056 x 3056
9.33 MPx
2048 x 2048
4.19 MPx
2048 x 2048
4.19 MPx
2048 x 2048
4.19 MPx
Quantum efficiency
(relevant to detect weak fluorescence)
~55 % ~70 % >80 % ~80 %
Light source High performance long-life LED High performance long-life LED High performance long-life LED High performance long-life LED
Illumination / Fluorescence1 Brightfield and 6 fluorescence excitation/emission channels Brightfield and 4 fluorescence excitation sources, up to 5 fluorescence emission filters Brightfield and 6 fluorescence
excitation/emission channels
Brightfield and 4 fluorescence excitation sources, up to 5 fluorescence emission filters
Temp.-control Possible3 x Possible3 x
Image acquisition- & device controlling software4 YT-Software®4 YT-Software®4 YT-Software®4 YT-Software®4
Automation-ready set up x5 x5
Software-interface for automation
Batch processing interface
External barcode-reader Optional Optional Optional Optional
  1. Different system configurations like e.g. basic systems with brightfield only or customized filter and objective lenses set-ups are available.
  2. Throughput per day depends on experiment configurations (e.g. exposure time, plate type, focus options, # of imaging channels…).
  3. Cellavista® can be equipped with the ibidi® heating and incubation system (other systems not tested). Due to pH-shift and consequent cell stress a heating option only is not recommended.
  4. YT-Software® includes the same image analysis tools for both CELLAVISTA® and NYONE®. Differences in usable applications arise only due to different hardware configurations (e.g. different illuminations or camera chips).
  5. Hardware automation-package available.

Key Applications in CLD

Cloning

Differences in throughput between CELLAVISTA® and NYONE® mainly arises due to the different chip size of the camera. Therefore a different number of images per well must be taken which, in turn, affects the measurement duration and the throughput:

Viability

The Trypan Blue viability test is a widely used technique to determine the cell number and the culture viability. SYNENTEC ported the classical Trypan Blue staining method to NyONE® and Cellavista® in a plate based approach. Both automated cell imaging systems will carry out the Trypan Blue assay as whole-well measurements in 96 well microplates with significantly increasing the throughput (5 minutes per 96 samples). This will dramatically reduce the time-to-result as well as costs. Both systems require a sample volume of just 20 µl which is ideal for a culture run dealing with low culture volume in total.

If the Trypan Blue viability assay is your main application, the NyONE® cell imager fits perfectly your needs!

CSMOS Camera Specification Imaging CLD

Viability Assay

The Trypan Blue viability test is a widely used technique to determine the cell number and the culture viability. SYNENTEC ported the classical Trypan Blue staining method to NYONE® and CELLAVISTA® in a plate based approach.

TryB breit png

Both automated cell imaging systems will carry out the Trypan Blue assay as whole-well measurements in 96 well microplates with significantly increasing the throughput (5 minutes per 96 samples). This will dramatically reduce the time-to-result as well as costs. Both systems require a sample volume of just 20 µl which is ideal for a culture run dealing with low culture volume in total.

If the Trypan Blue viability assay is your main application, the NYONE® cell imager fits perfectly your needs!

Key Applications in High-Content Imaging

Fluorescence Assays

The NYONE® is equipped with 4 FL excitation channels and with up to 6 detection wavelengths which covers approx. 80% of all FL dyes. (like Dapi®, eGFP, mCherry and Alexa®647). 3 different lenses (4x to 20x) allow many different methods to pick up FL signals which can be useful to gain throughput or to get a more detailed view inside of the cell.
The outstanding stitching quality of the NYONE® guarantees a non-overlapping full well imaging of your sample even by using the highest resolution of the 20x (0.53 µm/px). Weak FL signals will be detected due to the high power excitation in combination with the sensitive camera used for the NYONE® setup.

The CELLAVISTA® is equipped with 2 more fluorescent channels than the NyONE® and is therefore more versatile for fluorescence applications, especially ICC and IHC staining, due to the additional 40x magnification. Nevertheless most of the commonly used fluorophores like Dapi®, eGFP, mCherry and Alexa®647 can be measured with the NYONE®.

fluorescence-assays_1.png

iPS cells

Due to the high performance white light unit of the CELLAVISTA® sophisticated brightfield applications with weak contrast, such as iPS-cell characterization, are ideal for the CELLAVISTA® imaging system. Depending on your cell morphology and experimental composition the NyONE® is also sufficient for your stem cell application.

Compare Illumination Specifications

Excitation Source CELLAVISTA 4® HighEnd1 & Scientific NYONE® HighEnd1 & Scientific
UV (377/50 nm) Dapi, Hoechst…
Blue (475/28 nm) eGFP, Oyster®488, Calcein, Cy2…
Green (529/24 nm) 7-AAD… x
Lime (562/40 nm) mCherry, Cy3…, CellTracker® red
Amber (580/23 nm) Cy3, Rhodamine Phalloidin x
Red (632/22 nm) Cy5, Oyster®647…
Emission Filter
Blue (452/45 nm) Dapi, Hoechst…
Green (530/43 nm) eGFP, Oyster®488, Cy2…
RedLP (593/LP nm) mCherry, RFP, TRITC…
Amber (607/70 nm) 7-AAD…
Red (628/32 nm) Cy3, Rhodamine Phalloidin x
Far Red (685/40 nm) Cy5, Oyster®647…
Poly-Filter 1
(432/40 nm, 522/26 nm, 650/48 nm)
x
Poly-Filter 2
(471/19 nm, 530/20 nm)
x
Poly-Filter 3
(525/15 nm; 628/16 nm)
x
  1. Different system configurations like e.g. basic systems with brightfield only or customized filter and objective lenses set-ups are available

Our fluorescence viewer shows the differences in the fluorescent channels pretty good.

Cell cloning and upscaling are critical bottlenecks for biopharmaceutical R&D and cellular research. SYNENTEC's imaging systems support state-of-the-art automation and can be easily linked with:

  • Plate Stackers
  • Incubators
  • Liquid Handlers
  • Clone Pickers

Contact one of the following companies for further information:

Automation – Intelligent Handling Systems

PAA, Beckman Coulter, Tecan, Hamilton, Perkin Elmer, Thermo Fisher
Beckman Coulter Automation Cellavista
PAA Connect Automation NyONE