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High content analysis of tumor- & stromal-cell co-culture in ibidi inserts
Cell migration is very important for the invasion processes and metastasis of cancer cells. Here, tumor and stromal cells stained with various CellTracker ™ stains were cultured in μ-Dish containing a culture insert 4 well of ibidi. After removal of the insert, cell migration into the cell-free gap was periodically imaged with NYONE® Scientific SC4 Cell Imager. The cell-free gaps were found automatically and highly precisely using YT-software® and quantified after the measurements by the integrated image processing software.