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Automated Wound Healing and Migration assays

Cell migration is very important for the invasion processes and metastasis of cancer cells. Here, we demonstrate the basic principle of a wound healing, scratch and migration assays while using round and rectangular silicone inserts as well as handmade scratches while seeding the premalignant cell line KRAS. The removal of the silicone inserts and the “injury” caused by scratching created a cell-free gap. Cell migration into the cell-free gap was periodically imaged with the CELLAVISTA® imaging system and analyzed with the automated 2 phases wound healing operator of the YT-software®.

Scratch Assay_Time Line_Gap Closure