Cell Line Development
The development of a new cell line with the characteristics of interest is a highly complex process consisting of many successive steps, mainly leading into the goal to produce specific biopharmaceuticals.
Herein single cell cloning (SCC) represents a critical stage. The aim of SCC is to identify and isolate the most productive monoclonal cell populations after transfection or hybridization. Our Systems greatly provide the determination of monoclonality in different customizable approaches.
Furthermore controlling your transfection efficiency, determining the cell number, cell viability and the apoptosis rate e.g. in fed-batch cultures, detecting and counting colonies and quantifying IgG levels can be carried out reliably with SYNENTEC's imaging systems.
Characterization of pluripotency is an important aspect of stem cell research. Pluripotency refers to the potential of cells to differentiate it into any of the three germ layers. This property is shared by embryonic stem (ES) cells, embryonic germ (EG) cells and embryonic carcinoma (EC) cells.
Due to the rare availability, the ethical concerns and difficult cultivation, the investigation of induced pluripotent stem (iPS) cells is moving more and more into the focus of stem cell research. Like ES cells iPS cells are also pluripotent without having the disadvantages of ES cells.
Fluorescent labeled antibodies or colorimetric detection of pluripotency markers are provided with the brilliant optical resolution of SYNENTEC's imaging systems as well as validation of the proliferation and cell migration for wound healing assays.
Drug Discovery and Cancer Research
Cancer is no longer necessarily fatal, although still too many people die every year by the consequences of cancer. Research can help to fight against this insidious disease and diminishing the risks. The search for novel therapies against cancer is one of the most important areas of modern health research. But the diagnosis, prevention and aftercare must also be investigated. Having a fast and reliable way to perform in vitro pre-selections of individual compound collections is an important step which massively speeds up the entire process of drug discovery.
Especially in the sector of developing new diagnostic tools and also to understand the way of knock out mechanisms, CD-marker (cluster of differentiation) play an important role. Since CD-markers are cell type specific, there is a lot of ongoing research involved in the development of antibodies against CD-markers expressed by pathological cells (e.g. Rituximab®).
In the R&D-area of cellular immunology many subcategories can be divided – e.g. the infectiology, the study of immune deficiencies, the vaccine development, the development of therapeutic antibodies and the reproductive immunology.
All areas combines that complex cell assays have to be performed on mostly rare samples with expensive consumables. To ensure the accuracy and reproducibility of experiments and to save the samples, a fast and automated measurement and image analysis method should be selected.